Sayyed Hossein Hashemi; Fateme Keykha
Abstract
In this research a graphene oxide/zinc oxide nanocomposite (GO/ZnO) was synthesized and employed for simple and sensitive pipette tip-based micro-solid phase extraction (PT-µSPE) of nalidixic acid (NA) from seawater and human blood plasma samples following by its determination by spectrophotometry. ...
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In this research a graphene oxide/zinc oxide nanocomposite (GO/ZnO) was synthesized and employed for simple and sensitive pipette tip-based micro-solid phase extraction (PT-µSPE) of nalidixic acid (NA) from seawater and human blood plasma samples following by its determination by spectrophotometry. Several parameters affecting the suggested protocol were optimized, namely type and volume of eluent, amount of sorbent, sample volume, number of cycles of extraction and elution, pH of sample solution and type and amount of salt. The optimization performed by both response surface methodology (RSM) and one-variable-at-a-time techniques. Figures of merit were achieved as: detection limit; 0.30 µg L-1, enrichment factor 40 for NA, linear calibration curve in the range of 1.0-200.0 µg L-1; and reproducibility (as RSD %) better than 4.4%. Results for the application of the technique in seawater and human blood plasma showed that this analysis method can be applied for the determination of the analyte in complex real samples successfully.
Sayyed Hossein Hashemi; Massoud Kaykhaii; Ahmad Jamali Keikha; Enayat Saberi
Abstract
This paper describes trace determination of malachite green (MG) as a water pollutant dye by convenient spectrophotometry. A water-soluble hyper-branched polyamine was first prepared using the nuclophilic ring opening reaction of diepoxy and diamine monomer, which was then used for functionalization ...
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This paper describes trace determination of malachite green (MG) as a water pollutant dye by convenient spectrophotometry. A water-soluble hyper-branched polyamine was first prepared using the nuclophilic ring opening reaction of diepoxy and diamine monomer, which was then used for functionalization of multiwalled carbon nanotubes. This compound (named WHPA-OMCNT) was applied as a highly efficient adsorbent for the extraction of MG from seawater samples of Chabahar Bay (located in the southern east of Iran). WHPA-OMCNT was used in a pipette-tip solid phase extraction process; and for this extraction, different parameters affecting the extraction efficiency, including type and volume of eluent solvent, sample of volume, number of cycles of extraction and elution, pH of sample solution, type and amount of salt, and concentration of surfactant (triton X-114) were optimized using both one-variable-at-a-time and Box-Behnken response surface methodology techniques employing seven factors in three-levels. Under optimum conditions, the linear range of proposed method for MG was 4-250 µg L-1 with a detection limit of 0.80 µg L-1 and RSDs better than 6.4%.
