Mahnaz Ghereghlou; Jalil Afshar; Seyed Hossein Mousavi
Abstract
Measuring preservative concentrations in food is crucial due to their potential health implications. Therefore, it is necessary to develop a reliable and validated analytical method for monitoring. This study focuses on the validation of a simple, sensitive and precise analytical approach using HPLC-DAD ...
Read More
Measuring preservative concentrations in food is crucial due to their potential health implications. Therefore, it is necessary to develop a reliable and validated analytical method for monitoring. This study focuses on the validation of a simple, sensitive and precise analytical approach using HPLC-DAD to concurrently assess sodium benzoate and potassium sorbate. The validation process resulted in exceptional outcomes, demonstrating strong linearity (R2 > 0.999), precision (RSD < 5%), and accuracy (recovery values ranging from 90.77% to 100.55%). Additionally, the method exhibited low limits of detection and quantification (0.8 and 2.65 mg L-1 for sodium benzoate, and 0.14 and 0.47 mg L-1 for potassium sorbate, respectively). The effectiveness of the validated method on different food metrics was shown by analyzing 110 samples of Olivier salad, dairy products and ketchup sauce from Mashhad, Iran. Comparison of the results with Iran's national standards revealed that the preservative concentrations in most samples were within the acceptable limits set by Iranian regulations.
Mohammad Vahidifar; Zarrin Eshaghi; Ramin Rezaee
Abstract
In this paper, a new rapid and sensitive method based on sodium dodecyl sulfate modified Fe3O4@α–Linolenic acid nanocomposite combined with high-performance liquid chromatography-photo diode array detection (HPLC–PDA) has been proposed for the extraction and determination of tramadol ...
Read More
In this paper, a new rapid and sensitive method based on sodium dodecyl sulfate modified Fe3O4@α–Linolenic acid nanocomposite combined with high-performance liquid chromatography-photo diode array detection (HPLC–PDA) has been proposed for the extraction and determination of tramadol (TRA) in water samples. The Fe3O4@α–Linolenic acid NPs were synthesized and then characterized by Fourier transform-infrared spectroscopy (FT−IR), scanning electron microscopy (SEM) and X-ray diffraction (XRD). The main factors influencing extraction and desorption efficiency were optimized. Under optimum conditions, the method was successfully applied to the determination of TRA in the environmental samples and good linearity in the range of 0.1–500ng.mL-1(𝑅2> 0.99) obtained. The detection limit (LOD) and relative standard deviation (RSD) were0.074ng.mL-1 and 2.89 %( n=5) respectively. Finally, the proposed method was successfully applied with the relative recoveries percentages from 94–103.97% for the extraction and determination of tramadol in aqueous samples.
