Khanage Shantaram Gajanan; Dharam Prachi Gangadhar; Mohite Popat Baban; Deshmukh Vinayak Kashinath
Volume 2, Issue 2 , September 2015, , Pages 120-126
Abstract
A simple, sensitive, accurate and precise simultaneous UV spectrophotometric method has been developed for the estimation of Carisoprodol, Paracetamol and Caffeine in tablet dosage form. The absorption maxima of the drugs were found to be 221, 248 and 273 nm for Carisoprodol, Paracetamol and Caffeine ...
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A simple, sensitive, accurate and precise simultaneous UV spectrophotometric method has been developed for the estimation of Carisoprodol, Paracetamol and Caffeine in tablet dosage form. The absorption maxima of the drugs were found to be 221, 248 and 273 nm for Carisoprodol, Paracetamol and Caffeine respectively, in methanol, using a double beam UV/Visible Spectrophotometer. Carisoprodol, Paracetamol and Caffeine obeyed Beer’s law in the concentration range of 0.4-2 μg/mL, 2-10 μg/mL and 2-10 μg/mL, respectively. The correlation coefficient was found to be 0.9922, 0.9917 and 0.9943 for Carisoprodol, Paracetamol and Caffeine, respectively. The method was validated for various parameters according to International Conference on Harmonization guidelines. The low relative standard deviation values indicate a good precision and high recovery values indicate the accuracy of the proposed method.
Popat Baban Mohite; Shantaram Gajanan Khanage; Sandip Shankar Jadhav
Volume 2, Issue 1 , March 2015, , Pages 57-62
Abstract
Eperisone Hydrochloride is a skeletal muscle relaxant and Paracetamol is cyclooxygenase inhibitor. These drugs in combination are used as antispasmodics. A simple, specific, precise and accurate method was developed, namely reverse phase high performance liquid chromatography for simultaneous estimation ...
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Eperisone Hydrochloride is a skeletal muscle relaxant and Paracetamol is cyclooxygenase inhibitor. These drugs in combination are used as antispasmodics. A simple, specific, precise and accurate method was developed, namely reverse phase high performance liquid chromatography for simultaneous estimation of Eperisone Hydrochloride and Paracetamol in tablet dosage form. In RP-HPLC method separation was achieved by HiQ silC-18HS column having 250 mm× 4.6 mm, with mobile phase containing Methanol: 0.05 mM Ammonium acetate buffer: Acetonitrile (60:30:10) and adjusted to pH 5.8 using Glacial acetic acid for RP-HPLC system. The flow rate was 1.0 ml/min and effluent was monitored at 264 nm. The retention time of EPR and PAR were 6.45 min and 3.05 min respectively. The linearity for EPR and PAR were in the range of 5-25 µg/mL. The recoveries of EPR and PAR were found in the range of 99.96-100.52% and 99.87-100.11%, respectively. The proposed method was validated as per ICH guidelines and successfully applied to the estimation of EPR and PAR in tablet dosage form.
