Masoomeh Shaghaghi; Gholamreza Dehghan; Fatemeh Khajevand
Abstract
Chelation therapy has been used to remove toxic metals from the body for a long time. Flavonoids such as quercetin (QUR), a well-known protective antioxidant and free radical scavenger, can bind to metal cations and protect our bodies from toxic metals. In the current study, we used UV–Vis, 1HNMR, ...
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Chelation therapy has been used to remove toxic metals from the body for a long time. Flavonoids such as quercetin (QUR), a well-known protective antioxidant and free radical scavenger, can bind to metal cations and protect our bodies from toxic metals. In the current study, we used UV–Vis, 1HNMR, IR, and fluorescence spectroscopic techniques as well as viscosity measurements to investigate the synthesis, characterization, and interaction between Bi(III)-QUR complex and calf thymus DNA (ctDNA) in physiological buffer. The antioxidant activity of the complex was assessed utilizing DPPH and ABTS free radical scavenging, and ferric reducing potential. After chelation of the Bi(III) cation, the antioxidant potential of QUR was reduced. In the presence of ctDNA, the absorption spectrum of Bi(III)-QUR complex was raised, and the fluorescence intensity of Bi(III)-QUR complex was increased. With the addition of the Bi(III)-QUR complex, the relative viscosity of ctDNA rose. These findings indicate that the Bi(III)-QUR complex interacts with ctDNA in a groove-binding mode. The thermodynamic parameters (ΔH, ΔS, and ΔG) of the Bi(III)-QUR complex with ctDNA, as well as well as association constant, Ka, and number of binding sites (n), were assessed from the fluorescence data, indicating that the binding of Bi(III)-QUR complex to ctDNA was primarily driven by hydrophobic interactions.
Asghar Amiri; Mohammad Rahimipour
Volume 3, Issue 1 , March 2016, , Pages 38-41
Abstract
In this research, poly (DL-lactide-co-glycolide)-block-poly (ethylene glycol), (PLGA-PEG) nanoparticles (NPs) of less than 195 nm in diameter containing of Naringenin (NRG) a naturally flavonoid were synthesized. Encapsulated form NRG improves its medical properties and solubility. The therapeutic efficacy ...
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In this research, poly (DL-lactide-co-glycolide)-block-poly (ethylene glycol), (PLGA-PEG) nanoparticles (NPs) of less than 195 nm in diameter containing of Naringenin (NRG) a naturally flavonoid were synthesized. Encapsulated form NRG improves its medical properties and solubility. The therapeutic efficacy of the encapsulated naringenin (NRG-NPs) and NRG on human lung epithelial (A549) and mouse mammary (4T1) carcinoma cells proliferation was determined by MTT assays. The cytotoxicity potency was rated as follows: NRG-NPs > NRG. The antioxidant effects of the NRG and NRG-NPs were also determined by FRAP method. Our results show that NRG-NPs are cytotoxic compounds for cancer cells and anti-cancer effect can be attributed to the presence of Fe chelatory and antioxidant effects of NRG-NPs.
