TY - JOUR ID - 1619 TI - Reversed Phase High Performance Liquid Chromatographic Method for Simultaneous Estimation of Eperisone Hydrochloride and Paracetamol in Tablet Dosage Form JO - Iranian Journal of Analytical Chemistry JA - IJAC LA - en SN - 2383-2207 AU - Baban Mohite, Popat AU - Gajanan Khanage, Shantaram AU - Shankar Jadhav, Sandip AD - Department of Pharmaceutical Chemistry and PG Studies, M.E.S. College of Pharmacy, Sonai, Tq‐Newasa, Dist.‐Ahmednagar, Maharashtra, India‐414105. Y1 - 2015 PY - 2015 VL - 2 IS - 1 SP - 57 EP - 62 KW - Eperisone Hydrochloride KW - Paracetamol KW - RP-HPLC DO - N2 - Eperisone Hydrochloride is a skeletal muscle relaxant and Paracetamol is cyclooxygenase inhibitor. These drugs in combination are used as antispasmodics. A simple, specific, precise and accurate method was developed, namely reverse phase high performance liquid chromatography for simultaneous estimation of Eperisone Hydrochloride and Paracetamol in tablet dosage form. In RP-HPLC method separation was achieved by HiQ silC-18HS column having 250 mm× 4.6 mm, with mobile phase containing Methanol: 0.05 mM Ammonium acetate buffer: Acetonitrile (60:30:10) and adjusted to pH 5.8 using Glacial acetic acid for RP-HPLC system. The flow rate was 1.0 ml/min and effluent was monitored at 264 nm. The retention time of EPR and PAR were 6.45 min and 3.05 min respectively. The linearity for EPR and PAR were in the range of 5-25 µg/mL. The recoveries of EPR and PAR were found in the range of 99.96-100.52% and 99.87-100.11%, respectively. The proposed method was validated as per ICH guidelines and successfully applied to the estimation of EPR and PAR in tablet dosage form. UR - https://ijac.journals.pnu.ac.ir/article_1619.html L1 - https://ijac.journals.pnu.ac.ir/article_1619_cf107cd254cc17b121af3ae478eb68c4.pdf ER -