Document Type : Full research article

Authors

• Farideh Shamsi
• Ali Sheibani
• M. Reza Shishehbore

Department of Chemistry, Yazd Branch, Islamic Azad University, Yazd, Iran

Abstract

In this study, a dispersive magnetic solid phase extraction (DMSPE) was described by combining graphene oxide (GO) with Fe₃O₄ and CuO (Fe₃O₄@CuO&GO) for extraction and preconcentration of aspirin (ASP) in biological samples. The morphology and structure of the prepared nanocomposite was characterized and analyzed by XRD, SEM and FTIR techniques. Ion mobility spectrometry (IMS) method equipped to a corona discharge ionization source was exploited to determine ASA. The extraction parameters (desorption solvent, pH, adsorbent amount, extraction time and temperature) and also the operational parameters of IMS were investigated and optimized. Under the optimum conditions, the DMSPE–IMS method provided a linear range 6.0–40.0 ng for ASA with coefficient of determination R² = 0.99. The LOD and LOQ values were 0.9 and 3.0 ng for ASA, respectively. The repeatability of developed method was evaluated as relative standard deviation (RSD% = 2.7). The proposed method was also used to determine ASA in human plasma and serum as biological samples, which recovery results were within 89.0–100.0%.

Keywords

• Nanocomposite
• Dispersive magnetic solid phase extraction
• Aspirin
• Ion mobility spectrometry
• Biological samples