Neda Barekzahi; Niloufar Akbarzadeh-T
Abstract
In this project, in the first part, the Schiff base ligand (3-nitrobenzaldehyde + 2-aminothiophenol = H2L) was prepared. Then a nano-complex with the formula [Zn(HL)2Cl2] was synthesized using sonochemical method. Zn-nano-complex was identified by FT-IR, UV-Vis, 1H-NMR and Emission Scanning Electron ...
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In this project, in the first part, the Schiff base ligand (3-nitrobenzaldehyde + 2-aminothiophenol = H2L) was prepared. Then a nano-complex with the formula [Zn(HL)2Cl2] was synthesized using sonochemical method. Zn-nano-complex was identified by FT-IR, UV-Vis, 1H-NMR and Emission Scanning Electron Microscope (SEM). The spectroscopic results showed that the H2L ligand was coordinated to the metal through the N atom. The FT-IR spectrum of Zn-nano-complex showed the vibrational banb of the immunity group (C = N) at 1680-1600 cm-1. The studies obtained from the UV-Vis spectrum confirm n → π* and π→π* intra-ligand transitions. FS-DNA (salmon sperm DNA) and Zn-nano-complex were examined using UV-Vis spectroscopy, fluorescence spectroscopy, and gel electrophoresis. The Stern–Volmer equation was used to calculate the binding constant (Kb) and apparent bimolecular quenching constant (kq) for FS-DNA. Van der Waals forces and hydrogen bonds are important in the interaction between the DNA and the Zn (II) complex, according to an analysis of thermodynamic parameters (∆H°, ΔS° and ΔG°). The interaction mechanism was determined to be the groove-binding mode. These results highlight the complex's complicated effects on DNA structure and the need to use a variety of analytical techniques in order to fully investigate metal-DNA interactions.
