Hamzeh Adelpour Abdoli; Kamal Alizadeh; Payman Hashemi
Abstract
A magnet-enhanced solid-phase extraction technique utilizing spherical core–shell nanomagnetic agarose particles wasA magnet-enhanced solid-phase extraction technique utilizing spherical core–shell nanomagnetic agarose particles was devised to extract remdesivir from serum samples. These ...
Read More
A magnet-enhanced solid-phase extraction technique utilizing spherical core–shell nanomagnetic agarose particles wasA magnet-enhanced solid-phase extraction technique utilizing spherical core–shell nanomagnetic agarose particles was devised to extract remdesivir from serum samples. These nanomagnetic agarose particles underwent activation through the epichlorohydrin method and were subsequently modified using quercetin dihydrate as a ligand to facilitate remdesivir extraction. To quantify the target analyte, high-performance liquid chromatography (HPLC) was employed following preconcentration via the developed method. The influence of various analytical variables, including pH, ionic strength, magnet passes, and adsorbent quantity, was systematically examined and optimized using a multivariate central composite design approach. Under optimal conditions, five consecutive analyses demonstrated a remdesivir recovery rate of 99.4%, with a relative standard deviation of 3.66%. The method’s detection limit (3σ) for remdesivir was determined to be 0.027 mg L−1. This extraction technique was successfully validated for the quantification of remdesivir in serum samples
Mohammad Hassannejad; Kamal Alizadeh; Nemati Mahboob; Amir Abbas Matin; Roya Pourmohammad
Abstract
A simple, rapid, and cost-effective method for the determination of 17-β-estradiol in water samples was developed. The method is based on the extraction of the 17-β-estradiol–β-cyclodextrin complex using a coacervate phase composed of reverse micelles of decanoic acid, followed by ...
Read More
A simple, rapid, and cost-effective method for the determination of 17-β-estradiol in water samples was developed. The method is based on the extraction of the 17-β-estradiol–β-cyclodextrin complex using a coacervate phase composed of reverse micelles of decanoic acid, followed by high-performance liquid chromatography with ultraviolet detection for quantification. The effects of key parameters, including decanoic acid concentration (50–200 mg in a 30 mL total volume), tetrahydrofuran concentration (1–15% v/v), β-cyclodextrin to 17-β-estradiol molar ratio (1:1–1:4), ionic strength (0–1 M) NaCl, pH (1–4), and extraction time (0–30 min), on recoveries and enrichment factors were studied and optimized. The optimal extraction conditions involved stirring a 20 mL water sample containing 50 mg of decanoic acid with 3 mL of THF, using a 1:1 molar ratio of 17-β-estradiol to β-cyclodextrin complex, for 10 minutes, followed by centrifugation, 10 min at 4000 rpm. Recoveries and enrichment factors of 17-β-estradiol was primarily influenced by the decanoic acid and THF concentrations that form the coacervate phase but remained independent of the ionic strength of the sample solution. The recovery rate, enrichment factor, limit of detection, and relative standard deviation for 17-β-estradiol were 95%, 284, 0.19 μg/L, and 4.34%, respectively. This method was applied to analyze 17-β-estradiol in city water, mineral water, and pastewater samples. No 17-β-estradiol was detected in mineral water, while its concentration in city water and pastewater was found to be 1.9 μg/L and 33.67 μg/L, respectively.
