Daryoush Afzali; Elaheh Bagheri
Abstract
Monitoring the blood levels of phenylalanine, an essential amino acid, plays a vital role in the treatment of phenylketonuria. Cu-phthalocyanine (CuPC) and Au nanoparticles (AuNPs)-mediated non-enzymatic carbon paste electrode (CPE) has been developed for the electrochemical monitoring of L-phenylalanine ...
Read More
Monitoring the blood levels of phenylalanine, an essential amino acid, plays a vital role in the treatment of phenylketonuria. Cu-phthalocyanine (CuPC) and Au nanoparticles (AuNPs)-mediated non-enzymatic carbon paste electrode (CPE) has been developed for the electrochemical monitoring of L-phenylalanine (L-phe). The oxidation signal of L-phe was not observed on bare CPE or AuNPs/CPE. Although AuNPs was not involved in the apparition of L-phe oxidation peak, it enhanced the oxidation current. Using AuNPs-CuPC/CPE, we have successfully determined the different concentrations of L-phe with no need to any enzyme on the electrode surface. The performance characteristics of this sensor were accomplished with differential pulse anodic stripping voltammetry (DPASV) and cyclic voltammetry (CV). After optimizing the experimental parameters, L-phe gave a linear response over the concentration range of 1.0-130.0 µM with the detection limit of 0.41 µM. The practical applications of the modified electrode were demonstrated by measuring the concentration of L-phe in blood serum and urine samples.
