Daryoush Afzali; Elaheh Bagheri
Abstract
Monitoring the blood levels of phenylalanine, an essential amino acid, plays a vital role in the treatment of phenylketonuria. Cu-phthalocyanine (CuPC) and Au nanoparticles (AuNPs)-mediated non-enzymatic carbon paste electrode (CPE) has been developed for the electrochemical monitoring of L-phenylalanine ...
Read More
Monitoring the blood levels of phenylalanine, an essential amino acid, plays a vital role in the treatment of phenylketonuria. Cu-phthalocyanine (CuPC) and Au nanoparticles (AuNPs)-mediated non-enzymatic carbon paste electrode (CPE) has been developed for the electrochemical monitoring of L-phenylalanine (L-phe). The oxidation signal of L-phe was not observed on bare CPE or AuNPs/CPE. Although AuNPs was not involved in the apparition of L-phe oxidation peak, it enhanced the oxidation current. Using AuNPs-CuPC/CPE, we have successfully determined the different concentrations of L-phe with no need to any enzyme on the electrode surface. The performance characteristics of this sensor were accomplished with differential pulse anodic stripping voltammetry (DPASV) and cyclic voltammetry (CV). After optimizing the experimental parameters, L-phe gave a linear response over the concentration range of 1.0-130.0 µM with the detection limit of 0.41 µM. The practical applications of the modified electrode were demonstrated by measuring the concentration of L-phe in blood serum and urine samples.
Daryoush Afzali; Behnoosh Bahadori; Zahra Afzali
Abstract
A novel coronavirus (CoV), SARS-CoV-2 surfaced in late 2019 in Wuhan, China and spread across whole world. We started a study on COVID-19 and several clinical inhibitors, firstly we did molecular simulation on COVID-19 by Gromacs tools. Then simulated conformation was docked with suggested drugs for ...
Read More
A novel coronavirus (CoV), SARS-CoV-2 surfaced in late 2019 in Wuhan, China and spread across whole world. We started a study on COVID-19 and several clinical inhibitors, firstly we did molecular simulation on COVID-19 by Gromacs tools. Then simulated conformation was docked with suggested drugs for confirmation docking. The molecular docking results were similar to X-ray crystallography results in protein data bank and the analyses were confirmed by this method. The resulting conformation of the reported drugs with the COVID-19 was used for docking analyses. Furthermore, to study receptor conformation stability, a second MD simulation on complex was performed in an aqueous environment. RMSD for complex showed that the COVID19 conformation did not change in the presence of the suggested drugs. The results of docking showed that estimated free energy of binding, final intermolecular energy and hydrogen bond play an important role in interaction between suggested drugs and COVID-19. The results emerging docking showed that Sofosbuvir, vitamin D and 2aurintricarboxylic acid have been potential to be applied as new COVID-19 anti corona virus drugs.
Maryam Akhondi; Daryoush Afzali; Ali Mostafavi
Abstract
In this study, iron oxide/cellulose acetate nanocomposite was prepared by electrospinning method and its performance was evaluated for removal of arsenic. Here, oleic acid coated magnetite nanoparticle was synthesized by co-precipitation method and was impregnated in cellulose acetate solution. This ...
Read More
In this study, iron oxide/cellulose acetate nanocomposite was prepared by electrospinning method and its performance was evaluated for removal of arsenic. Here, oleic acid coated magnetite nanoparticle was synthesized by co-precipitation method and was impregnated in cellulose acetate solution. This magnetite impregnated polymer solution, was electrospun to form nanocomposite polymer fiber. The cellulose acetate/iron oxide nanocomposite membrane was characterized by SEM and FTIR. The magnetic properties of composite nanofibers were characterized by using vibrational sample magnetometry analysis. The nanocomposite was used to remove arsenic ions from aqueous solution. Batch adsorption experiments were carried out to study the sorption behavior of arsenic ions as a function of pH, contact time and initial concentration. Experimental results showed that the maximum capacity of the cellulose acetate-iron oxide nanocomposite membrane for removal of arsenic from low concentration is 0.36 mg/g at pH 9. For better investigation of the adsorption mechanism, two isotherm models, Langmuir and Freundlich were tested. Based on the isothermal results, adsorption data were fitted well to Langmuir isotherm. The reusability of the nanocomposite membrane was confirmed for several adsorption and desorption processes by acid-alkali treatment.
