Document Type : Full research article
Authors
1 Faculty of Chemistry, Lorestan University, Khorramabad 6813717133, Iran
2 Faculty of Chemistry, Lorestan University, Khorramabad, 68137-17133 Iran
Abstract
A magnet-enhanced solid-phase extraction technique utilizing spherical core–shell nanomagnetic agarose particles was
A magnet-enhanced solid-phase extraction technique utilizing spherical core–shell nanomagnetic agarose particles was devised to extract remdesivir from serum samples. These nanomagnetic agarose particles underwent activation through the epichlorohydrin method and were subsequently modified using quercetin dihydrate as a ligand to facilitate remdesivir extraction. To quantify the target analyte, high-performance liquid chromatography (HPLC) was employed following preconcentration via the developed method. The influence of various analytical variables, including pH, ionic strength, magnet passes, and adsorbent quantity, was systematically examined and optimized using a multivariate central composite design approach. Under optimal conditions, five consecutive analyses demonstrated a remdesivir recovery rate of 99.4%, with a relative standard deviation of 3.66%. The method’s detection limit (3σ) for remdesivir was determined to be 0.027 mg L−1. This extraction technique was successfully validated for the quantification of remdesivir in serum samples
Keywords
[1] K. Wu, D. Su, J. Liu, R. Saha and J.P. Wang, Magnetic nanoparticles in nanomedicine: a review of recent advances. J. Nanotechno. 30 (2019) 502003.
[2] G.F. Stiufiuc and R.I. Stiufiuc, Magnetic nanoparticles.Magnetic Nanoparticles: Synthesis, Characterization. J. Biomedical Field Appl. Sci. 14 (2024) 1623.
[3] Y. Wang, I. Nkurikiyimfura and Z.H. Pan, Sonochemical Synthesis of Magnetic Nanoparticles. Chem. Eng. Commun. 202 (2015) 615-621.
[4] M. Ansari, M.M. Kadhim, B.A.Hussein, H.A. Lafta and E. Kianfar, Synthesis and stability of magnetic nanoparticles. Bionanosci. 12 (2022) 627-638.
[5] E.M. Kostyukhin and L.M. Kustov, Microwave-assisted synthesis of magnetite nanoparticles possessing superior magnetic properties. Mendeleev Commun. 28 (2018) 559-561.
[6] P. Zarrintaj,S. Manouchehri, Z. Ahmadi, M.R. Saeb, A. Urbanska, D.L Kaplan and M. Mozafari, Agarose-based biomaterials for tissue engineering. Carbohydr. Polym. 187 (2018) 66-84.
[7] H. Tabani, M. Alexovi, J. Sabo and M. R. Payan, An overview on the recent applications of agarose as a green biopolymer in micro-extraction-based sample preparation techniques. Talanta 224 (2021) 121892.
[8] J. Yin, H. Zheng, H. Lin, J. Sui, B. Wang, T. R. Pavase and L. Cao, Boronic acid functionalized agarose affinity chromatography for isolation of tropomyosin in fishes. J. Sci. Food Agric. 99 (2019) 6490-6499.
[9] M.R. Green and J. Sambrook, Agarose gel electrophoresis. Cold Spring Harb. Protoc. 1 (2019) 87-95.
[10] M.K. Yazdi, A. Taghizadeh, M. Taghizadeh, F.J Stadler, M. Farokhi, F. Mottaghitalab, P. Zarrintaj, J.D. Ramsey, F. Seidi ,M.R. Saeb and M. Mozafari, Agarose-based biomaterials for advanced drug delivery. J. Control. Release 326 (2020) 523-543.
[11] A.S. Amin, H.H. El-Feky, and N. Hassan, A novel sensor for the selective monitoring of trace ytterbium ions using an agarose-based optical membrane. RSC adv. 12 (2022) 26620-26629.
[12] A.L. Capriotti, C. avaliere, G. La, B. Carmela, M. Montone, S. Piovesana and A. Lagan, Recent applications of magnetic solid-phase extraction for sample preparation. Chromatographia 82 (2019) 1251-1274.
[13] L. Chen, Y. He, Zh. Lei, C. Gao, Q. Xie, P. Tong and Z. Lin, Preparation of core-shell structured magnetic covalent organic framework nanocomposites for magnetic solid-phase extraction of bisphenols from human serum sample. Talanta 181 (2018) 296-304.
[14] M. Safari, M. Shahlaei, Y. Yamini, M. Shakorian and E. Arkan, Magnetic framework composite as sorbent for magnetic solid phase extraction coupled with high performance liquid chromatography for simultaneous extraction and determination of tricyclic antidepressants. Anal. Chim. Acta 1034 (2018) 204-213.
[15] M. Hemmati, M. Rajabi, and A. Asghari, Magnetic nanoparticle based solid-phase extraction of heavy metal ions: a review on recent advances. Mikrochim. Acta 185 (2018) 1-32.
[16] T.D. Ho, A.J. Canestraro and J. Anderson, Ionic Liquids in Solid-Phase Microextraction: A Review. Anal. Chim. Acta 695 (2011) 18-43.
[17] M. Hosseini, A. Rezaei and M. Soleymani, Homogeneous solvent‑based microextraction method(HSBME) using a task‑specific ionic liquid and its application to the pectrophotometric determination of fluoxetineas pharmaceutical pollutant in real water and urine samples. Chem. Pap. 78 (2024) 8195–8210.
[18] M. Hosseini, R. Castillo and M. Soleymani, A novel magnetic-assi
[19] J. Ma, G. Wu, S. Li, W Tan, X. Wang, J. Li and L. Chen, Magnetic solid-phase extraction of heterocyclic pesticides in environmental water samples using metal-organic frameworks coupled to high performance liquid chromatography determination. J. Chromatogr. A 1553 (2018) 57-66.
[20] A.H. Bakheit, H. Darwish, I.A. Darwish and A.I. Al-Ghusn, Remdesivir. Profiles Drug Subst. Excip. Relat. Methodol. 48 (2023) 71-108.
[21] J.H. Beigel, K.M. Tomashek, L.E. Dodd, A.K. Mehta, B.S. Zingman, A.C. Kalil and E. Hohmann, Remdesivir for the treatment of Covid-19. N. Engl. J. Med. 383 (2020) 1813-1826.
[22] J.J. Malin, I. Suárez, V. Priesner, G. Fätkenheuer and J. Rybniker, A comparative study of HPLC and UV spectrophotometric methods for remdesivir quantification in pharmaceutical formulations. J. Taibah Univ. Sci. 15 (2021) 507-513.
[23] J.D. Ruos, M.A. Baldo and S. Daniele, Analytical methods for the determination of major drugs used for the treatment of COVID-19. A review CRC 53 (2023) 1698-1732.
[24] P. Du, G. Wang, S. Yang, P. Li and L. Liu, Quantitative HPLC-MS/MS determination of Nuc, the active metabolite of remdesivir, and its pharmacokinetics in rat. J. Anal. Bioanal. Chem. 413 (2021) 5811–5820.
[25] M.E. Sharkasy, M.M. Tolba, F. Belal, M.I Walash and R. Aboshabana, Simultaneous spectrofluorimetic determination of remdesivir and simeprevir in human plasma. Sci. Rep. 12 (2022) 21980.
[26] M. Shohan, R. Nashibi, M.R. Mahmoudian Sani, F. Abolnezhadian, M. Ghafourian, S. M. Alavi, A. Sharhaniand A. Khodadadi, The therapeutic effcacy of quercetin in combination with antiviral drugs in hospitalized COVID-19 patients: A randomized controlled trial. European J. Pharmacol. 914 (2022) 1-8.
[27] M. Hassannejad, K. Alizadeh, M. Nemati, A.A. Matin and R. pourmohammad, Extraction and quantification of 17-β-Estradiol in wastewater samples via coacervative phase formation with β-Cyclodextrin and high-performance liquid chromatography. Iran J. Aal. Chem. 11(2) (2024) 173-181.
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