نوع مقاله : مقاله پژوهشی کامل
نویسندگان
1 بخش شیمی، کالج علوم طبیعی، دانشگاه جیما، جیما، اتیوپی
2 بخش شیمی، کالج علوم طبیعی، دانشگاه جیما، جیما، اتیوپی؛ آژانس توسعه علم وفناوری دولت منطقه ای ارومیا، آدیس آبابا، اتیوپی
چکیده
در این مطالعه، ورتکس یاریشده با دانسیته پایین بر اساس میکرواستخراج مایع-مایع پخشی و دنبالشده با کروماتوگرافی مایع با کارآیی بالا توسط آشکارساز ماوراء بنفش برای تعیین آفتکش های کلرفلورنل-متیل، کلرفنوینفس و دی آزینون در نمونههای آبهای محیطی بکار گرفته شد. پارامترهای مختلف مؤثر بر راندمان استخراج، شامل نوع و حجم حلال استخراجی و پخشکننده pH نمونه، اثر نمک، زمان سانتریفیوژ و ورتکس بررسی گردید و شرایط بهینه بدست آمد. تحت شرایط بهینه، منحنیهای کالیبراسیون در محدوده غلظتی 100-5/8، 100-5/3 و 600-5/36 نانوگرم بر میلیلیتر برای سه آفتکش نامبرده با ضریب همبستگی (r2) برابر با 993/0 یا بهتر خطی بودند. حدود تشخیص و تعیین آنالیتها بر اساس 3 و 10 برابر نسبت سیگنال به نویز به ترتیب در محدوده 11-9/0 و 8/36-1/3 نانوگرم بر میلی لیتر محاسبه گردید. روش پیشنهادی بطور موفقیتآمیزی برای آنالیز نمونههای آب بکار برده شد. بازیابیهای نسبی (RR%) با اضافه کردن دو سطح غلظت در محدوه %108-79 و با انحراف استانداردهای نسبی (RSD%) در رنج %9/9-1/9 مطالعه گردید. نتایج این مطالعه اثبات میکندکه روش پیشنهادی برای استخراج یا پیش تغلیظ سه آفتکش قبل از اندازهگیری کمی توسط دستگاه HPLC–UV/V مؤثر میباشد.
کلیدواژه‌ها
[1] M.C.R. Alavanja, Pesticides use and exposure extensive worldwide, Rev. Environ. Health. 24 (2009) 303–309.
[2] WHO/FAO. International code of conduct on the distribution and use of pesticides: guidelines for the registration of pesticides, Rome, Italy. 2010.
[3] F.P. Garcia, S.Y.C. Ascencio, J.C.G. Oyarzun, A.C. Hernandez and P.V. Alavarado, Pesticides: classification, uses and toxicity. Measures of exposure and genotoxic risks, Int. J. Environ. Sci. Toxic. Res. 1 (2012) 279–293.
[4] M. Galrilescu, Review: Fate of pesticides in the environment and its bioremediation, Eng. Life Sci. 5 (2005) 497–526.
[5] A.d.S. Pinheiro and J.B.d. Andrade, Development, validation and application of a SDME/GC-FID methodology for the multiresidue determination of organophosphate and pyrethroid pesticides in water, Talanta 79 (2009) 1354–1359.
[6] C.A. Damalas and I.G. Eleftherohorinos, Pesticide Exposure, Safety Issues, and Risk Assessment Indicators, Int. J. Environ. Res. Public Health 8 (2011) 1402–1419.
[7] WHO. Organophosphorous pesticides in the environment-integrated risk assessment, Geneva: 2001.
[8] S. Wang, B. Xiang and Q. Tang, Trace determination of dichlorvos in environmental samples by room temperature ionic liquid-based dispersive liquid-phase microextraction combined with HPLC, J. Chromatogr. Sci. 50 (2012) 702–708.
[9] T. Bedassa, A. Gure and N. Megersa, Low density solvent based dispersive liquid ⎯liquid microextraction and preconcentration of multiresidue pesticides in environmental waters for liquid chromatographic analysis, J. Anal. Chem. 70 (2015) 1199–1206.
[10] Q. Zhou, H. Bai, G. Xie and J. Xiao, Trace determination of organophosphorus pesticides in environmental samples by temperature controlled ionic liquid dispersive liquid-phase microextraction, J. Chromatogr. A 1188 (2008) 148–153.
[11] T. Tolcha, Y. Merdassa and N. Megersa, Low-density extraction solvent based solvent-terminated dispersive liquid–liquid microextraction for quantitative determination of ionizable pesticides in environmental waters, J. Sep. Sci. 36 (2013) 1119–1127.
[12] C. Wang, Q. Wu, C. Wu and Z. Wang, Determination of some organophosphorus pesticides in water and watermelon samples by microextraction prior to highperformance liquid chromatography, J. Sep. Sci. 34 (2011) 3231–3239.
[13] A. Cappiello, G. Famiglini and P. Palma, Trace level determination of organophosphorus pesticides in water with the new direct-electron ionization LC/MS interface. Anal. Chem. 74 (2002) 3547–3554.
[14] N. Dujaković, S. Grujić, M. Radišić, T. Vasiljević and M. Laušević, Determination of pesticides in surface and ground waters by liquid chromatography-electrospray–tandem mass spectrometry, Anal. Chim.Acta 678 (2010) 63–72.
[15] C. Crescenzi, A. Di Cocia, E. Gurrriero, and R. Samperi, Development of a multiresidue method for analyzing pesticide traces in water based on solid-phase extraction and electrospray liquid chromatography mass spectrometry, Environ. Sci. Technol. 31 (1997) 479-488.
[16] M.A. Farajzadeh, S.E. Seyedi, M.S. Shalamzari and T.M. Bamorowa, Dispersive liquid–liquid microextraction using extraction solvent lighter than water. J. Sep. Sci. 32 (2009) 3191–3200.
[17] P.G. Su, and S.D. Huang, Determination of organophosphorus pesticides in water by solid-phase microextraction, Talanta 49 (1999) 393–402.
[18] B. Albero, C. Sanchez-Brunete and J.L. Tadeo, Determination of organophosphorus pesticides in fruit juices by matrix solid-phase dispersion and gas chromatography. J. Agri. Food Chem. 51 (2003) 6915–6921.
[19] F. Rodrigues, P. Mesquita, L. Oliveira, F. Oliveira, A. Filho, A. Pearo and J. Andrae, Development of head space solid-phase microextraction/gas chromatography–mass spectrometry method for determination of organophosphorus pesticide residues in cow milk. Microchim. J. 98 (2011) 56–61.
[20] M. Rezaee, Y. Assadi, M. Hosseini, E. Aghaee, F. Ahmadia and S. Berijani, Determination of organic compounds in water using dispersive liquid–liquid microextraction. J. Chromatogr. A. 1116, (2006) 1–9.
[21] J. Ma, R. Xiao, J. Li, X. Zhao, B. Shi and S. Li, Determination of organophosphorus pesticides in underground water by SPE-GC–MS, J. Chromatogr. Sci.47 (2009) 111-115.
[22] T.A. Albanis and D.G. Hela, Multi-residue pesticide analysis in environmental water samples using solid-phase extraction discs and gas chromatography with flame thermionic and mass-selective detection, J. Chromatogr. A 707 (1995) 283–292.
[23] H. Çabuk, M. Akyüz and Ş. Ata, A simple solvent collection technique for a dispersive liquid–liquid m icroextra ction of parabens from aqueous samples using low-density organic solvent, J. Sep. Sci. 35 (2012) 2645–2652.
[24] P. Soisungnoen, R. Burakham and S. Srijarana Determination of organophosphorus pesticides using dispersive liquid–liquid microextraction combined with reversed electrode polarity stacking mode -micellar electrokinetic chromatography, Talanta 98 (2012) 62–68.
[25] L. Zhou, Z. Luo, S. Wang, Y. Hui, Z. Hu and X. Chen, In-capillary derivatization and laser-induced fluorescence detection for the analysis of organophosphorus pesticides by micellar electrokinetic chromatography, J. Chromatogr. A, 1149 (2007) 377–384.
[26] H. C. Liang, N. Bilon and M. T. Hay, Analytical methods for pesticide residues, Water Environ. Res. 85 (2013) 2114-2138.
[27] Y. Chen, Z. Guo, X. Wang and C. Qiu, Review: Sample preparation, J. Chromatogr. A 1184 (2008) 191–219,
[28] T. Padrón, A. Olivares, C. Ferrera and Z. Rodríguez, Microextraction techniques coupled to liquid chromatography with mass spectrometry for the determination of organic micro pollutants in environmental water samples, Molecules 19 (2014) 10320-10349.
[29] C. Nerín, J. Salafranca, M. Aznar and R. Batlle, Critical review on recent developments in solventless techniques for extraction of analytes, Anal. Bioanal. Chem. 393 (2009) 809–833
[30] M. Rezaee, Y. Yamini and M. Faraji, Evolution of dispersive liquid–liquid microextraction method, J. Chromatogr. A 1217 (2010) 2342–2357.
[31] L. Kocúrová, I.S. Balogh, J. Šandrejová and, V. Andruch, Recent advances in dispersive liquid–liquid microextraction using organic solvents lighter than water. A review, Microchem. J. 102 (2012) 11–17.
[32] A. Gure, F.J. Lara, A.M. García-Campaña, N. Megersa, and M. del Olmo-Iruela, Vortex-assisted ionic liquid dispersive liquid–liquid microextraction for the determination of sulfonylurea herbicides in wine samples by capillary high-performance liquid chromatography, Food Chem. 170 (2015) 348–353.
[33] C. Wu, H. Liu, W. Liu, Q. Wu, C.H. Wang and Z. Wang, Determination of organophosphorus pesticides in environmental water samples by dispersive liquid–liquid microextraction with solidification of floating organic droplet followed by high performance liquid chromatography, Anal. Bioanal. Chem., 397 (2010) 2543–2549.
[34] C.K. Zacharis, P.D. Tzanavaras, K. Roubos and K. Dhima, Solvent-based de-emulsification dispersive liquid–liquid microextraction combined with gas chromatography–mass spectrometry for determination of trace organochlorine pesticides in environmental water samples. J. Chromatogr. A 1217 (2010) 5896–5900.
[35] M.I. Leong and S.D. Huang, Dispersive liquid–liquid microextraction method based
on solidification of floating organic drop for extraction of organochlorine pesticides in water samples, J. Chromatogr. A 1216 (2009) 7645–7650.
on solidification of floating organic drop for extraction of organochlorine pesticides in water samples, J. Chromatogr. A 1216 (2009) 7645–7650.
[36] H., Chen, R., Chena and S. Li, Low-density extraction solvent-based solvent terminated dispersive liquid-liquid microextraction combined with gas chromatography-tandem mass spectrometry for the determination of carbamate pesticides in water samples. J. Chromatogr. A 1217 (2010) 1244–1248.
[37] L. Guo and H.K. Lee, Low-density solvent based ultrasound-assisted emulsification microextraction and on-column derivatization combined with gas chromatography–mass spectrometry for the determination of carbamate pesticides in environmental water samples, J. Chromatogr. A 1235 (2012) 1235, 1–9.
[38] D. Moreno González, L. Gámiz Gracia, J.M. Bosque Sendraand and A.M. GarcíaCampaña, Dispersive liquid-liquid microextraction using a low density extraction solvent for the determination of 17 N-methylcarbamates by micellar electrokinetic chromatography-electrospray-mass spectrometry employing a volatile surfactant, J. Chromatogr. A 1247 (2012) 26–34.
[39] Z. Yang, Y. Lu, Y. Liu, T. Wu, Z. Zhou and D. Liu, Vortex-assisted surfactantenhanced emulsification liquid–liquid microextraction, J. Chromatogr. A 1218 (2011) 7071–7077.
[40] K. Seebunrueng, Y. Santaladchaiyakit and S. Srijaranai, Vortex-assisted low density solvent based demulsified dispersive liquid–liquid microextraction and high-performance liquid chromatography for the determination of organophosphorus pesticides in water samples, Chemosphere 103 (2014) 51–58.
[41] EUROPEAN COMMISSION: Guidance document on analytical quality control and method validation procedures for pesticides residues analysis in food and feed. SANTE/11945/2015; Supersedes SANCO/12571/2013, Implemented by 01/01/2016.
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