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Selective Determination of Glucose in Blood Plasma by Using an Amperometric Glucose Biosensor Based on Glucose Oxidase and a Chitosan/ Nafion/ IL/Ferrocene Composite Film

Document Type : Full research article

Authors

Electroanalytical Chemistry Research Lab., Faculty of Sciences, Azerbaijan Shahid Madani University, P.O. Box: 53714-161, Tabriz, Iran

Abstract

 
A kind of ferrocene mediated enzymatic biosensor was fabricated in order to the measurement of glucose as an important biological analyte. A homogenous mixture of ferrocene including Chitosan(CS), Nafion(Nf) and an imidazolium based ionic liquid, 1-butyl-3-methyl imidazoliumhexafluorophosphate, [BMIM]HF6PO4, was prepared. The mentioned mixture was cast on the surface of carbon ceramic electrode and an enzymatic layer was entrapped between two layers of this mixture in order to improving the mechanical stability of the biosensor. Cyclic voltammetry and chronoamperometry were used to investigate the analytical performance of the biosensor. Remarkable deduction of interfering signals produced by AA and UA was obtained by applying a constant potential (180mV) on the human plasma for a short period of time (100min). Values of practical parameters such as sensitivity (2.73 µA mM-1cm-2), linear range (95.23–1367.5 µM) and detection limit (48 µM) were obtained by the chronoamperometric studies. The apparent Michaels-Menten constant, Km (3.52 mM) was also calculated. In order to estimate the practical accuracy and precision of the enzymatic biosensor, a test of spiked glucose solution recovery in natural plasma was done.

Keywords

References
 
[1]     V. Pishko, I. Katakis, S. E. Lindquist, L.Ye, B. A. Gregg and A. Heller, Direct Electrical Communication between Graphite Electrodes and Surface Adsorbed Glucose Oxidase/Redox Polymer Complexes, Angew. Chem. Int. Ed. Engl. 29 (1990) 82–84.
[2]     L. C. Clark and Jr. Ch. Lyons, Electode Systems For Continiouse Mnitoring In Cardiovascular  Surgery, Ann. NY Acad. Sci. 102 (1962) 29-45.
[3]     S. Updike and G. Hicks, Enzyme electrode, Nature 214 (1967) 986–988.
[4]     G.G. Guilbault and G.J. Lubrano, An enzyme electrode for the amperometric determination of glucose, Anal. Chim. Acta 64 (1973) 439-455.
[5]     A. Albisser, B. Leibel, T. G. Ewart, Z . Davidovac, C. Botz and W. Zingg, An ArtificialEndocrine Pancreas, Diabetes 23 (1974) 389-396.
[6]     M. Shichiri,Y. Yamasaki, R. Kawamori, N. Hakui and H. Abe, Wearable, Artificial Endoctrine Pancreas With Needle–Type Gucose Sensor, Lancet. 320 (1982) 1129-1131.
[7]     A.E.G. Cass, G. Davis, G.D. Francis, H.A.O. Hill, W.J. Aston, I.J. Higgins, E.V. Plotkin, L.D.L. Scott and A.P.F. Turner, Ferrocene-mediated enzyme electrode for amperometric determination of glucose, Anal. Chem. 56 (1984) 667–671.
[8]     Y. Degani and A. Heller, Direct electrical communication between chemically modified enzymes and metal electrodes, Phys. Chem. 91 (1987) 1285–1289.
[9]     T.J. Ohara, R. Rajagopalan and A. Heller, Wired Enzyme Electrodes for Amperometric Determination of Glucose or Lactate in the Presence of Interfering Substances, Anal. Chem. 66 (1994) 2451–2457.
[10] A. Heller, Electrical wiring of redox enzymes, Acc. Chem. Res. 23 (1990) 128–134.
[11] L. Rabinovich and O. Lev, Sol-Gel Derived Composite Ceramic Carbon Electrodes, Electroanal. Chem. 13 (2001) 265–275.
[12] P. Audebert, S. Sallard, and S. Sadki, Electrochemical Investigation on the Polycondensation Kinetics of Silicon Alkoxides by Functionalization of the Silica Network by Redox Species, Phys. Chem. 107 (2003) 1321–1325.
[13] P. Audebert ,G. Cerveau, R.J.P. Corriu and N. Costa, Modified electrodes from organic-inorganic hybrid gels formed by hydrolysis- polycondensation of some trimethoxysilylferrocenes, Electro. anal. Chem. 413 (1996) 89-96.
[14] T.J.M. Luo, R. Soong, E.Lan, B. Dunn and C. Montemagno, Photo-induced proton gradients and ATP biosynthesis produced by vesicles encapsulated in a silica matrix, Nat. Mater 4 (2005) 220-224.
[15] J.D. Brennan, Biocomposites: Using light to drive biosynthesis, Nat. Mater 4 (2005)189–190.
[16] H. Ju, X. Zhang , J. Wang , Nanobiosensing Principles, Developments and Applications ,Springer science and Business media, (2011), New York , pp.151-154.
[17] V. S. Elanchezhian,  M. Kandaswamy, A ferrocene-based multi-signaling sensor molecule functions as a molecular switch, Inorg. Chem. Commun 12 (2009) 161–165
[18] J. D. Qiu, W. Mei Zhou, J. Guo, R. Wang and R.P. Liang, Amperometric sensor based on ferrocene-modified multiwalled carbon nanotube nanocomposites as electron mediator for the determination of glucose, Anal. Biochem. 385 (2009) 264-269.
[19] J.B. Raoof, R. Ojani and M. Kolbadinezhad, Voltammetric sensor for glutathione determination based on ferrocene-modified carbon paste electrode, Solid State Electrochem. 13 (2009) 1411-1416.
[20] H. Zhou, W. Yang and C. Sun, Amperometric sulfite sensor based on multiwalled carbon nanotubes/ferrocene-branched chitosan composites, Talanta 77 (2008) 366-371.
[21] G. ChaoZhao, M. QingXu and Q. Zhang, A novel hydrogen peroxide sensor based on the redox of ferrocene on room temperature ionic liquid film, Electrochem. Commun. 10 (2008) 1924-1926.
[22] S. Pandey, Analytical applications of room-temperature ionic liquids: A review of recent efforts, Anal. Chim. Acta 556 (2006) 38-45.
[23] P. Yu, Y. Lin,L. Xiang, L. Su, J. Zhang and L. Mao, Molecular Films of Water-Miscible Ionic Liquids Formed on Glassy Carbon Electrodes:  Characterization and Electrochemical Applications, Langmuir 21 (2005) 9000–9006.
[24] Y. Zhao, H. Liu, Y. Kou, M. Li , Z. Zhu and Q. Zhuang, Structural and characteristic analysis of carbon nanotubes-ionic liquid gel biosensor, Electrochem. Commun. 9 (2007) 2457-2462.
[25] J.D. Wadhawan,U. Schroder,A. Neudeck,  S.J. Wilkins, R.G. Compton, F. Marken, C.S.Consorti, R.F. Souza and J. Dupont, Ionic liquid modified electrodes. Unusual partitioning and diffusion effects of Fe(CN)64−/3− in droplet and thin layer deposits of 1-methyl-3-(2,6-(S)-dimethylocten-2-yl)-imidazolium tetrafluoroborate, Electroanal. Chem. 493 (2000) 75-83.
[26] F. Zhao, X.Wu, M. Wang, Y. Liu, L. Gao and S. Dong, Electrochemical and Bioelectrochemistry Properties of Room-Temperature Ionic Liquids and Carbon Composite Materials, Anal. Chem. 76 (2004) 4960–4967.
[27] Q. Wang, H. Tang, Q. Xie, L. Tan, Y. Zhang, B. Li and S. Yao, Room-temperature ionic liquids/multi-walled carbon nanotubes/chitosan composite electrode for electrochemical analysis of NADH, Electrochimica. Acta 52 (2007)6630-6637.
[28] M.C. Buzzeo, C. Hardacre, and R.G. Compton, Use of Room Temperature Ionic Liquids in Gas Sensor Design, Anal. Chem. 76 (2004) 4583–4588.
[29] S.F. Ding, M.Q. Xu, G.C.Zhao and X.W. Wei, Direct electrochemical response of Myoglobin using a room temperature ionic liquid, 1-(2-hydroxyethyl)-3-methyl imidazolium tetrafluo-roborate, as supporting electrolyte, Electrochem. Commun. 9 (2007) 216-220.
[30] M. Tsionsky, G. Gun, V. Glezer, and O. Lev, Sol-Gel-Derived Ceramic-Carbon Composite Electrodes: Introduction and Scope of Applications, Anal. Chem 66 (1994) 1747–1753.
[31] T. Kong, Y.Chen,Y.Ye, K. Zhang, Z. Wang,  X. Wang, An amperometric glucose biosensor based on the immobilization of glucose oxidase on the ZnO nanotubes, Sens. Actuators. 138 (2009) 344-350.
[32] S.C. Kou,B.J. Cherayil,W. Min,B.P. English, and X.S. Xie, Single-Molecule Michaelis −Menten Equations, Phys. Chem. 109 (2005) 19068–19081.
[33] L.Yang, X. Ren, F.Tang and L. Zhang, A practical glucose biosensor based on Fe3O4 nanoparticles and chitosan/nafion composite film, Biosens. Bioelectron. 25 (2009) 889-895.
Iranian Journal of Analytical Chemistry
Volume 5, Issue 1 - Serial Number 1
March 2018
Pages 9-16
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