In collaboration with Payame Noor University and Iranian Chemical Science and Technologies Association

Document Type : Full research article

Authors

Department of Chemistry, Omidiyeh Branch, Islamic Azad University, Omidiyeh, Iran

Abstract

Fluorescent chemical sensors to detect drugs, by increasing fluorescence emission and absorption or by shutting down, because they are non-destructive, the ability to show decomposed concentrations, fast response, high accuracy have been considered and used. In this research, a chemical sensor was synthesized PbS functionalized with gelatin quantum dots for (MNZ) drug. The calibration curve was linear in the range of (0.1 to 10.0 µg L−1). The standard deviation of (3.5%), and detection limit of the method (2.2 µg L−1 in time 50 sec, 285 nm) were obtained for sensor level response PbS Quantum Dot–Gelatin Nano composites sensor with (95%) confidence evaluated. The observed outcomes confirmed the suitability recovery and a very low detection limit for measuring the (MNZ) drug. The method fluorometric introduced to measure (MNZ) drug in real samples such as urine and blood was used and can be used for hospital samples. The chemical PbS Quantum Dot–Gelatin Nano composites sensor made it possible as an excellent sensor with good reproducibility.

Keywords

 
[1]     H. Shemer, Y.K. Kunukcu and K.G. Linden, Degradation of the pharmaceutical metronidazole via UV, Fenton and photo-Fenton processes. Chemosphere. 63 (2006) 269.
[2]      S. Benitez–Martinez, A.I. Lopez-Lorente and M. Valcarcel, Multilayer grapheme-gold nanoparticle hybrid substrate for the sers determination of metronidazole. Micro. Chem. J. 121 (2015) 6.
[3]     J. Muller, P. Schildknecht and N. Muller, Metabolism of nitro drugs metronidazole and nitazoxanide in Giardia lamblia: characterization of a novel nitroreductase. J. Antimicrob. Chemoth. 68 (2013) 1781.
[4]     J. Han, L. Zhang, S. Yang, J. Wang, A highly sensitive metronidazole sensor based on a Pt nanospheres/polyfurfural film modified electrode. J. Environ. Contam. Tox. 92 (2014) 196.
[5]     W. Jin, W. Li, Q. Xu, Q. Dong, Quantitative assay of metronidazole by capillary zone electrophoresis with amperometric detection at a gold microelectrode. J. Electrophoresis. 21(7) (2000) 1409-1414.
[6]     M. Yang, M. Guo, Y. Feng, Y. Lei, Y. Cao, D. Zhu, Y. Yu and L. Ding, Sensitive Voltammetric Detection of Metronidazole Based on Three - Dimensional Graphene - Like Carbon Architecture / Polythionine Modified Glassy Carbon Electrode. 165 (2018) 687.
[8]     S. Ashour and N. Kattan, Simultaneous determination of miconazole nitrate and metronidazole in different pharmaceutical dosage forms by gas chromatography and flame ionization detector (GC-FID). Int. J. Bio. Sci. 6 (2010) 13.
[9]     M. Silva, S. Schramm, E. Kano, E. Koono, V. Porta and C. Serra, Development and validation of a HPLC-MS-MS method for quantification of  metronidazole in human plasma. J. Chromatogr. 47(9) (2009) 781-784.
[10] N.W. Ali, M. Gamal and M. Abdelkawy, Chromatographic methods for simultaneous determination of diiodohydroxyquinoline and metronidazole in their binary mixture. J. Pharm. Sci. 26 (2013) 865.
[11] S.N. Makhija and P.R. Vavia, Stability indicating HPTLC method for the simultaneous determination of pseudoephedrine and cetirizine in pharmaceutical formulations. J. Pharm. Biomed. Anal. 25 (2001) 663.
[12] H. Liu, F. Li, R. Yang, L. Wang and Y. Ma, Determination of common antibiotics and metronidazole in cosmetics by ultraperformance liquid chromatography tandem mass spectrometry. J. Chinese. Chromatography. 27 (2009) 50.
[13] S.B. Wankhede, K.A. Lad and S.S. Chitlange, Development and validation of UV‑spectrophotometric methods for simultaneous estimation of cetirizine hydrochloride and phenylephrine hydrochloride in tablets. Int. J. Pharm. Sci. Drug. Res. 4 (2012) 222.
[14] N. Samadi and S. Narimani, Sensitive and Selective Determination of Metronidazole Using Highly Luminescent Pepper Carbon Dots. J. Chem. Biolog. Phys. Sci. 6 (2016) 387.
[15] E. Roy, S.K. Maity, S. Patra and P.K. Sharma, A metronidazole-probe sensor based on imprinted biocompatible nanofilm for rapid and sensitive detection of anaerobic protozoan. Adv. 4 (2014) 32881.
[16] J. Das and M. Dhua, UV-Spectrophotometric Assay Method Development and Validation of Metronidazole in Bulk and Tablet Formulation. J. Pharma. Sci. Tech. 3 (2014) 106.
[17] Y. Dai, K. Yao, J. Fu, K. Xue, L. Yang and K. Xu, A novel 2-(hydroxymethyl) quinolin-8-ol-based selective and sensitive fluorescence probe for Cd2+ ion in water and living cells. Sensor. Actuators B. 251 (2017) 877.
[18] W.B. Huang, W. Gu, H.X. Huang, J.B. Wang, W.X. Shen, Y.Y. Lv and J. Shen, A porphyrin-based fluorescent probe for optical detection of toxic Cd2+ ion in aqueous solution and living cells. Dyes Pigments. 143 (2017) 427.
[19] N.B. Brahim, N.B.H. Mohamed, M. Echabaane, M. Haouari, R.B. Chaabane, M. Negrerie and H.B. Ouada, Thioglycerol-functionalized CdSe quantum dots detecting cadmium ions. Sens. Actuators B. 220 (2015) 1346.
[20] A. Hatamie, F. Marahel and A. Sharifat, Green synthesis of graphitic carbon nitride nanosheet (G-C3N4) and using it as a label-free fluorosensor for detection of metronidazole via quenching of the fluorescence, Talanta. 176 (2018) 518.
[21] A. Tadesse, D. RamaDevi, M. Hagos, G.R. Battu and K. Basavaiah, Facile Green Synthesis of Fluorescent Carbon Quantum Dots from Citrus Lemon Juice for Live Cell Imaging. Asian. J. Nanoscience and Materials. 1(2018) 36-46.
[22] C. Knoblauch, M. Griep and C. Friedrich, Recent advances in the field of bionanotechnology: An insight into optoelectric bacteriorhodopsin, quantum dots, and noble metal nanoclusters. Sensors. 14 (2014) 19731.
[23] M. Mirsalari, S. Elhami, Colorimetric detection of insulin in human Serum using GO/AuNPs/TX100 nanocomposite. J. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 240 (2020) 118617.
[25] C.M. Kaye, M.J. Sankey and L.A. Thomas, A rapid and specific semi-micro method involving high-pressure liquid chromatography for the assay of metronidazole in plasma, saliva, serum, urine and whole blood, J. Clin. Pharmacology, 9 (1980) 528.
[26] A. Shokrollahi, H.E. Haghighi, E. Niknam and K. Niknam, Application of cloud point preconcentration and flame atomic absorption spectrometry for the determination of cadmium and zinc ions in urine, blood serum and water samples. J. Quim. Nova. Sao. Paul.  36 (2013) 273.
[27] C. Coester, K. Langer, H. Brisen and J. Kruter, Gelatin nanoparticles by two step desolvation-a new preparation method, surface modifications and cell uptake, J. Micro. encapsul. 17 (2000) 187.
[28] N. Samadi and S. Narimani, Citrate Capped CdS Quantum Dots as Fluorescence Sensor for Simple and Selective Determination of Metronidazole. Sensor. Lett. J. 14 (2016) 530.
[29] Y. Zhao, J. Zou and W. Shi, In situ synthesis and characterization of lead sulfide nanocrystallites in the modified hyperbranched polyester by gamma-ray irradiation, J. Mater. Sci. Eng. 121 (2005) 20.
[30] S. Lee, S. Cho and J. Cheon, Anisotropic shape control of colloidal inorganic nanocrystals, Adv. Mater. 15 (2003) 441.
[31] T.S. Shyju, S. Anandhi, R. Sivakumar and R. Gopalakrishnan, Studies on Lead Sulfide (PbS) Semiconducting Thin Films Deposited from Nanoparticles and Its NLO Application. Int. J. Nano. Sci. 13 (2014) 1450001.
[32] M. Zohreh, S.M. Ghoreishi, M. Behpour and M. Mohammadhassan, Applied electrochemical biosensor based on covalently self‐assembled monolayer at gold surface for determination of epinephrine in the presence of ascorbic acid. Arab. J. Chem. 10 (2017) 657–664.
[33] R. Adel, S. Ebrahim, A. Shokry, M. Soliman and Marwa Khalil, Nanocomposite of CuInS/ZnS and Nitrogen-Doped Graphene Quantum Dots for Cholesterol Sensing, ACS Omega. 6 (2021) 2167-2176.
[34] T.D. Thanh, J.  Balamurugan, N.T.  Tuan, H.  Jeong, S.H.  Lee, N.H.  Kim and J.H.  Lee, Enhanced electrocatalytic performance of an ultrafine AuPt nanoalloy framework embedded in graphene towards Epinephrine sensing. J. Biosens. Bioelectron. 89 (2016) 750.
[35] S.S. Liang, L. Qi, R.L. Zhang, M. Jin and Z.Q. Zhang, Ratiometric fluorescence biosensor based on CdTe quantum and carbon dots for double strand DNA detection. Sens. Actuators. 244 (2017) 585.
[36] P. Dutta, D. Saikia, N.C. Adhikary and N.S. Sarma, Macromolecular Systems with MSA-Capped CdTe and CdTe/ZnS Core/Shell Quantum Dots as Superselective and Ultrasensitive Optical Sensors for Picric Acid Explosive. ACS Appl. Mater. Interfaces. J. 7 (2015) 24778.
[37] S. Baluta, K. Malecha, A. Swist and J. Cabaj, Fluorescence Sensing Platforms for Epinephrine Detection Based on Low Temperature Cofired Ceramics. Sensors. 20 (2020) 1429.