نوع مقاله : مقاله پژوهشی کامل
نویسندگان
گروه شیمی، دانشکده علوم پایه، دانشگاه پیام نور، تهران، ایران
چکیده
درکار حاضر، الکترو اکسیداسیون اسید اسکوربیک و اسید فولیک، به عنوان دو ویتامین ضروری، در سطح الکترود کربن سرامیک اصلاح شده توسط پلی دوپامین و مس (Cu/PDA/CCE) بررسی شد. پلی دوپامین با استفاده از شرایط رسوبگذاری الکتروشیمیایی تهیه گردید. مشخصه یابی مقدماتی الکتروشیمیایی برای مطالعه رفتار الکترود ساخته شده برای اندازه گیری همزمان این دو مولکول زیستی انجام شد. با مطالعات ولتامتری الکترود تهیه شده، دو پیک آندی مجزا برای اسید فولیک و اسید اسکوربیک برای تشخیص همزمان ترکیبات پیدا شد. از رسم نمودارهای کالیبراسیون محدوده های خطی به ترتیب در دامنه 0.5 تا 360 میکرومولار و 0.83 تا 380 میکرومولار با حد تشخیص های به ترتیب 0.031 و 0.057 میکرومولاربرای اسیدفولیک و اسید اسکوربیک بدست آمدند. از کاربرد الکتروداصلاح شده نتایج رضایت بخشی در آنالیزنمونه ادرار حاصل شد.
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