Document Type : Full research article

Authors

• Farzaneh Marahel ¹
• Abdolmohsen Amouri ¹
• Alireza Geramizadegan ²
• Mohammad Reza Asghariganjeh ¹

¹ Department of Chemistry, Omidiyeh Branch, Islamic Azad University, Omidiyeh, Iran.

² Department of Chemistry, Dashtestan Branch, Islamic Azad University, Dashtestan, Iran.

Abstract

In this study, a description of spectrofluorometer method for the measure of epinephrine (EP) drug in urine and blood samples using lead sulfide (PbS) quantum dots via glutathione nanocomposite as a sensor using a resonance Rayleigh scattering (RRS) technique. The sensor was characterized using FTIR, XRD, SEM, and TEM. The scattering intensity (∆IRRS) signal was detected by a fluorescence detector at λ(ex) = 325 nm. Under the optimum conditions, the calibration plot is linear in the (EP) drug concentration range of (0.050‒200.0 ng L-1). The standard deviations of (1.2 %), detection limit (LOD) of the method (0.015 ng L-1) and quantification (LOQ) of the method (0.044 ng L-1) in time 50 s, 325 nm for sensor level response PbS QDs-glutathione nanocomposite with (96 %) confidence evaluated. Moreover, a PbS QDs-glutathione nanocomposite sensor with RRS technique for the analysis of (EP) drug in urine and blood samples with high % recoveries (94.0 -99.2 %), low % RSD values (< 3 %) was used. This method offers a reliable approach for detecting various drugs in clinical and pharmaceutical settings.

Keywords

• Epinephrine drug
• Glutathione
• PbS quantum dots
• Sensor
• Resonance Rayleigh Scattering